Alpha-1 Antitrypsin testing in Quebec can be easily accessed trough the 2 designated centers:

  • Centre Hospitalier de l'Université de Montréal (CHUM)

  • McGill University Health Center (MUHC)

Once the genetic testing process is initiated, all of the required samples will be collected up-front. Depending on the chosen testing center, samples will be proceed through a full integrated algorithm consisting of a serum level test and/or confirmatory testing (if a low serum level has been detected). Upon completion, a detailed report will be sent back to the requesting physician outlining the test results and interpretation.


 

CHUM NOTRE-DAME HOSPITAL - DR JEAN-PIERRE ÉMOND

Step 1:  A laboratory testing requisition form (Dr Émond website of CHUM laboratories). Fill in the sections below:

  • Patient information

  • Requesting physician information

  • Description of services required - please indicate DDAAT (Integrated protocol for the screening of Alpha-1 Antitrypsin Deficiency)

  • If serum levels are known, please indicate the result on the requisition form

Hospital or community laboratory should process samples as follows:

  • 2 tubes of frozen serum (Golden Cap -  5 ml - Serum decanted and shipped frozen)

  • 1 tube of Whole Blood (EDTA tube Purple/Lavender, - 4 ml , shipped at 4 °C, on ice)

Step 2: Include a copy of the laboratory requisition form and shipped samples to:

CHUM - Hôpital Notre-Dame
Pavillon Deschamps, Laboratoires 6e étage
1560 rue Sherbrooke Est
Montréal, Qc  H2L 4M1
Tél : (514) 890-8000 poste 25787


 

The details of the testing algorithm set up by Notre-Dame Hospital's laboratory (Dr Jean-Pierre Émond) can be viewed here. Turnaround time for the test results is between 7 and 90 days depending on the required confirmatory ALPHA-1 tests.

  • 7 days for serum AAT levels + HR-SPE alone

  • 30 days for serum AAT levels + HR-SPE + genotype

  • 90 days for phenotype AAT

*Although levels above 1.15 g/L are considered normal, serum levels below 1.15 g/L should be referred for confirmatory (qualitative) testing in order to capture as many borderline results as possible.


 

MUHC LABORATORY ROYAL-VICTORIA - DR BRIAN GILFIX

Step 1: Before genetic testing, it is highly recommended to determine AAT serum level into your local hospital or community laboratory. If the AAT serum levels are less than 1.15 g/L, confirmatory testing (PCR, isoelectric focusing and gene sequencing) can be carried out to confirm diagnosis*.

If you are unable to previously determine the AAT serum level, please go directly to Step 2.

Step 2:  Send your patient to your local hospital or community laboratory with the following:

FOR PHYSICIANS WITHIN MUHC

A laboratory testing requisition form. Complete the sections below :

  • Patient information

  • Requesting physician information

  • Description of services required - please indicate "Alpha-1 Antitrypsin serum level and Genetic Testing" OR "Alpha-1 Antitrypsin Genetic Testing" (if serum levels are known and less than 1.15 g/L)

  • If serum levels are known, please indicate the result on the requisition form


 

FOR PHYSICIANS OUTSIDE MUHC

Any other convenient form (including prescription pads) with the following detailed information:

  • Patient information

  • Requesting physician information

  • Description of services required - please indicate "Alpha-1 Antitrypsin serum level and Genetic Testing" OR "Alpha-1 Antitrypsin Genetic Testing" (if serum levels are known and less than 1.15 g/L)

  • If serum levels are known, please indicate the result on the requisition form. 

Hospital or community laboratory should process samples as follows:

  • 1 vial of frozen serum (2mL, shipped frozen)

  • 1 tube of whole blood (EDTA tube; purple cap, minimum 2mL, shipped at 4 ºC)

Step 3: Include a copy of the completed laboratory requisition form and shipped samples to:

Dr. Brian Gilfix
MUHC-Royal Victoria Hospital

Central Reception, C-6
687 Pine Avenue West
Montreal, Quebec H3A 1A1
Phone: 514-934-1934 x 34410


 

Details of the testing algorithm set up by Royal-Victoria's laboratory (Dr Brian Gilfix) can be viewed here.  Turnaround time for the test results is between 13 and 21 days.

*Although levels above 1.15 g/L are considered normal, serum levels below 1.15 g/L should be referred for confirmatory testing in order to capture as many borderline results as possible.